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DMGregory
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You can force Unity to run a single instance of your application by ticking Force Single Instance in Edit > Project Settings > Player > Resolution and Presentation

Screenshot of Project Settings window showing where to find the option

You can force Unity to run a single instance of your application by ticking Force Single Instance in Edit > Project Settings > Player

You can force Unity to run a single instance of your application by ticking Force Single Instance in Edit > Project Settings > Player > Resolution and Presentation

Screenshot of Project Settings window showing where to find the option

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Siruj
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You can force Unity to run a single instance of your application by ticking Force Single Instance in Edit > Project Settings > Player