After the @DMGregory♦ comment I come across [this](https://docs.unity3d.com/ScriptReference/EditorUtility.SetDirty.html) documentation. So, to have the changes made through the editor API serialized correctly I did change this way: Undo.RecordObject(gameObjectInScene, "Set reference for itsField."); gameObjectInScene.itsField = theFieldValue; PrefabUtility.RecordPrefabInstancePropertyModifications(gameObjectInScene);